Hepatocyte nuclear factor 1β gene regulation in E14.5 ureteric bud cells. Hepatocyte nuclear factor 1β gene regulation in E14.5 ureteric bud cells

HNF-1β mutations are one of the most common single-gene mutations that underlie kidney developmental disease. Hepatocyte nuclear factor 1β (HNF-1β) is essential for kidney development, but its functions in ureteric bud (UB) branching morphogenesis are incompletely understood. We isolated E14.5 UB cells using fluorescence-activated cell sorting, and performed RNA-sequencing to compare gene expression in wild-type and HNF-1β deficient UB cells. 1632 genes have significantly lower expression in the HNF-1β deficient UB cells and 2223 genes have significantly higher expression in HNF-1β deficient UB cells. Overall design: RNA-sequencing (RNA-seq) to compare gene expression in wild-type and HNF-1β deficient E14.5 ureteric bud cells

HNF-1β突变是引发肾脏发育疾病最常见的单基因突变之一。肝细胞核因子1β（Hepatocyte nuclear factor 1β，HNF-1β）对肾脏发育至关重要，但其在输尿管芽（ureteric bud，UB）分支形态发生中的功能尚未完全阐明。本研究通过荧光激活细胞分选术分离了胚胎发育第14.5天（E14.5）的输尿管芽细胞，并开展RNA测序（RNA-sequencing，RNA-seq）以比较野生型与HNF-1β缺陷型输尿管芽细胞的基因表达情况。在HNF-1β缺陷型输尿管芽细胞中，共有1632个基因的表达水平显著下调，另有2223个基因的表达水平显著上调。实验整体设计：通过RNA测序（RNA-seq）比较野生型与HNF-1β缺陷型胚胎发育第14.5天输尿管芽细胞的基因表达差异。