Datasets for the effects of RUNX2 silencing on transcriptomic and metabolomic profiles in SJSA-1 osteosarcoma cells. Datasets for the effects of RUNX2 silencing on transcriptomic and metabolomic profiles in SJSA-1 osteosarcoma cells

Osteosarcoma is the most common primary malignant bone tumor with a high risk of metastasis and recurrence. Metabolic reprogramming is a hallmark of osteosarcoma and other cancers and is associated with genetic and epigenetic alterations. RUNX2 is an important transcription factor for osteoblastic differentiation, and aberrant expression of the gene contributes to the development and progression of osteosarcoma. To identify the effects of RUNX2 silencing on transcriptomic and metabolomic profiles in osteosarcomas, we generated SJSA-1 osteosarcoma cells stably expressing RUNX2 shRNA and SJSA-1 cells stably expressing scramble shRNA and analyzed transcriptome and metabolome profiles in the two cell types using Illumina NovaSeq 6000 and ultrahigh-performance liquid chromatography coupled with time-of-flight mass spectrometry, respectively. The datasets can be used by researchers to identify novel targets of RUNX2 and elucidate the role and underlying mechanism of RUNX2 in osteosarcoma pathogenesis and metabolic reprogramming. Overall design: Comparing the mRNA profilings of SJSA-1 cells stably expressing RUNX2 shRNA or scramble shRNA.

骨肉瘤（Osteosarcoma）是最常见的原发性恶性骨肿瘤，具有较高的转移与复发风险。代谢重编程是骨肉瘤及其他癌症的标志性特征，与遗传及表观遗传改变密切相关。RUNX2是成骨细胞分化的关键转录因子，该基因的异常表达可促进骨肉瘤的发生与进展。为明确RUNX2沉默对骨肉瘤转录组与代谢组谱的调控效应，本研究构建了稳定表达RUNX2短发夹RNA（short hairpin RNA，shRNA）的SJSA-1骨肉瘤细胞系，以及稳定表达乱序shRNA的SJSA-1对照细胞系；并分别采用Illumina NovaSeq 6000测序平台与超高效液相色谱-飞行时间质谱联用技术，对两种细胞的转录组及代谢组谱进行分析。本数据集可供研究者用于挖掘RUNX2的潜在调控靶点，阐明RUNX2在骨肉瘤发生发展及代谢重编程过程中的作用与分子机制。整体实验设计：比较稳定表达RUNX2 shRNA与乱序shRNA的SJSA-1细胞的mRNA表达谱。