Gene expression analysis of wild-type and Tafazzin-deficient granulocyte-monocyte progenitor (GMP) clones (ER-HOXB8 conditionally immortalized)

Evaluate changes in gene expression resulting from the loss of the gene TAZ (protein tafazzin). Tafazzin is an enzyme involved in cardiolipin maturation and cardiolipin is a phospholipid found only in the inner mitochondrial membrane. Gene expression profiling was performed in a murine granulocyte-monocyte progenitor in vitro cell system. We identified a limited number of changes in gene expression, which was not surprising given that the tafazzin protein is localized in the mitochondria and has a very specific enzymatic activity. Genes involved in cholesterol and fatty acid metabolism (Acss1, Acat2, Ldlr), apoptosis (Bcl2, Casp3, Casp6, Cycs), and ER-stress induced apoptosis (Ddit3, Atf4) were differentially expressed (with a fold change ≥ 1.2 and p value < 0.05).

本研究旨在探究TAZ基因（tafazzin蛋白）缺失所诱导的基因表达变化。该tafazzin蛋白是一种参与心磷脂成熟过程的酶，而心磷脂是一种仅存在于线粒体内膜的磷脂。本研究在小鼠粒-单核细胞祖细胞体外培养体系中开展了基因表达谱分析。我们仅检测到少量基因表达发生改变，这一结果并不意外，因为tafazzin蛋白定位于线粒体且具有高度特异性的酶促活性。参与胆固醇与脂肪酸代谢的基因（Acss1、Acat2、Ldlr）、细胞凋亡相关基因（Bcl2、Casp3、Casp6、Cycs）以及内质网应激诱导的细胞凋亡相关基因（Ddit3、Atf4）均出现了差异表达（倍数变化≥1.2且P值<0.05）。