opt-SNE datasets

Data files referenced within the opt-SNE preprint (https://doi.org/10.1101/451690) are available for download belowFlow18parameter18 parameter fluorescent flow cytometry file from http://doi.wiley.com/10.1002/cyto.a.22983.flow18_annotated.fcs - an FCS file with all original channels. An extra channel is included that identifies cell types assigned by expert gating. The data are compensated and all fluorescent parameters scaled by the function asinh(x/150).flow18_for_optsne.csv - the data from above but converted to a CSV file that can be used as input to the open-source Multicore-opt-SNE package. Only channels used for opt-SNE analysis are included in the file. Note that the events are sorted by class ID, so if subsetting the file it is recommended to randomize the row order beforehand.tsne_results_flow18.csv - an example of opt-SNE results for the Flow18parameter dataset.Mass41parameter41 parameter mass cytometry dataset from https://doi.org/10.1126/science.1198704.mass41_annotated.fcs - an FCS file produced by concatenation of multiple files from the original dataset. An extra channel is included that identifies cell types assigned by expert gating. An additional extra channel identifies file of origin. All mass parameters are scaled by the function asinh(x/5).mass41_for_optsne.csv - the data from above but converted to a CSV file that can be used as input to the open-source Multicore-opt-SNE package. Only channels used for opt-SNE analysis are included in the file. Note that the events are sorted by class ID, so if subsetting the file it is recommended to randomize the row order beforehand.tsne_results_mass41.csv - an example of opt-SNE results for the Mass41parameter dataset.

opt-SNE预印本（https://doi.org/10.1101/451690）中引用的数据文件可于下方获取。源自http://doi.wiley.com/10.1002/cyto.a.22983的Flow18 18参数荧光流式细胞术数据集相关文件如下：
flow18_annotated.fcs：一款包含全部原始通道的FCS（Flow Cytometry Standard，流式细胞术标准文件格式）文件，新增了一个用于标识经专家门控分析确定的细胞类型的额外通道。数据已完成荧光补偿，所有荧光参数均经asinh(x/150)函数缩放。
flow18_for_optsne.csv：上述数据转换为CSV格式的文件，可作为开源Multicore-opt-SNE工具包的输入文件。该文件仅包含opt-SNE分析所需的通道。请注意，数据事件已按类别ID排序，因此若需对该文件进行子集截取，建议预先对行顺序进行随机化处理。
tsne_results_flow18.csv：Flow18参数数据集的opt-SNE分析结果示例文件。

源自https://doi.org/10.1126/science.1198704的Mass41 41参数质谱流式细胞术数据集相关文件如下：
mass41_annotated.fcs：一款由原始数据集多个文件合并得到的FCS文件，新增了两个额外通道：一个用于标识经专家门控分析确定的细胞类型，另一个用于标识数据来源文件。所有质谱参数均经asinh(x/5)函数缩放。
mass41_for_optsne.csv：上述数据转换为CSV格式的文件，可作为开源Multicore-opt-SNE工具包的输入文件。该文件仅包含opt-SNE分析所需的通道。请注意，数据事件已按类别ID排序，因此若需对该文件进行子集截取，建议预先对行顺序进行随机化处理。
tsne_results_mass41.csv：Mass41参数数据集的opt-SNE分析结果示例文件。