Figure_5_images_for_paper

(G-I) Viral RNA was extracted from viral stocks (G), Xrn1 knock out A549 cells 16 hours post-infection (H), or 48-hour supernatants from infected MDCK cells (H). Viruses analyzed:  WT PR8 and PR8-PA(∆X) strains with no insert, or PR8 with WT or mutant STOML2 sequences inserted in the WT PR8 or PR8-PA(∆X) background. Mutant STOML2 sequences contain the GCUG to UAGC mutation preventing PA-X cleavage. Viral RNA was reverse-transcribed and PCR-amplified using primers shown as black arrows in F-G to determine whether the STOML2 sequences were retained. Gel images representative of 3 experiments with separate virus rescues. Additional replicates can be found in Figure_5_replicates dataset.

R# --> replicate number; LG### --> experiment number

.sgd are the original source files obtained with the Syngene Imager

(G-I) 从病毒原液（viral stocks）、感染后16小时的Xrn1敲除A549细胞（Xrn1 knock out A549 cells），或感染MDCK细胞48小时的细胞上清液中提取病毒RNA（viral RNA）。本次分析的病毒毒株包括：无插入片段的野生型PR8与PR8-PA(∆X)毒株，或是在野生型PR8或PR8-PA(∆X)遗传背景中插入野生型或突变型STOML2序列的PR8毒株。其中突变型STOML2序列携带GCUG至UAGC的突变，可阻断PA-X的切割作用。将提取的病毒RNA进行反转录，并使用F-G图中标注为黑色箭头的引物进行PCR扩增，以检测STOML2序列是否得以保留。本次展示的凝胶图像为3次独立病毒拯救实验的代表性结果，其余重复实验数据可于Figure_5_replicates数据集中获取。

R# 代表重复样本编号；LG### 代表实验编号。

.sgd为通过Syngene成像仪（Syngene Imager）获取的原始源文件