Spiral ganglion subtype specification occurs prior to birth in the mouse

Little is known about the timing and factors which regulate the specification of neuronal subtypes in the cochlear spiral ganglion. Here we collect Spiral Ganglion Neurons (SGNs) from the mouse across four developmental timepoints (E14, E16, E18 and P1) in order to transciptionally profile their development. We dissected mouse cochleas at E14,E16, E18 and P1, removing as much non-neuronal tissue as possible, and pooling samples from 5 different embryos/pups. The tissue was dissociated and single SGNs captured and transcriptionally profiled using 10x Chromium and Illumina sequencing platforms. At E16 the spiral ganglion was cut in half and collected in apical and basal sections. Three individuall biological replicates were collected at each age, except P1 where 2 replicates were collected

目前学界对于耳蜗螺旋神经节神经元亚型特化的调控时序与调控因子尚所知甚少。本研究采集了四个发育时间点（E14、E16、E18及P1）的小鼠螺旋神经节神经元（Spiral Ganglion Neurons，SGNs），以对其发育过程进行转录组谱分析。我们在上述四个发育时间点解剖小鼠耳蜗，尽可能去除非神经元组织，并将5只不同胚胎/幼崽的样本进行混合。随后对组织进行解离，利用10x Chromium与Illumina测序平台捕获单个SGN并开展转录组谱分析。在E16时间点，我们将螺旋神经节对半切开，分别收集顶区与基区样本。每个发育时间点均设置3次生物学重复，仅P1时间点设置2次生物学重复。