Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube. Danio rerio

RNA-seq transcriptome analysis identified a functional requirement for zebrafish Rfx4 in the developing neural floor plate and roof plate. Overall design: Embryos derived from an rfx4uw8013/+ incross were sorted by phenotype into mutant and sibling groups. RNA was prepared from each individual embryo at ~ 25 hpf

RNA测序（RNA-seq）转录组分析鉴定出，斑马鱼Rfx4基因在发育中的神经底板与神经顶板中具有功能必需性。整体实验设计：由rfx4uw8013/+ 杂合互交产生的胚胎，通过表型特征被分为突变体组与同胞野生型组；于约25个受精后小时（hpf）时，从每个单个胚胎中提取RNA。