Bacterial Biogeography of Coastal Water in the Northern East China Sea. marine metagenome

The Northern East China Sea is a unique marine ecosystem with multiple environmental gradients (e.g. salinity, pH, and nutrients) along northwest to southeast, forming a horn-shaped pattern with Hangzhou Bay as the tip. This study applied 16S amplicon high-throughput sequencing with Illumina MiSeq technique and multiple statistical analyses to quantitatively reveal the relative contributions of local environmental conditions and regional spatial factors in shaping bacterial biogeographic pattern of coastal water using 95 surface water samples collected from 95 sites across 8 coastal zones, from Hangzhou Bay (northwest) to outside of Sanmen Bay (southeast), in the Northern East China Sea across a > 200 km scale.

东海北部是一处独特的海洋生态系统，其西北至东南方向存在盐度、pH值及营养盐等多重环境梯度，并以杭州湾为顶点形成角状分布格局。本研究采集了东海北部8个沿海区域内95个采样点的95份表层水样，采样范围覆盖从杭州湾（西北）至三门湾外侧（东南）的超200千米海域，采用基于Illumina MiSeq平台的16S扩增子高通量测序（16S amplicon high-throughput sequencing）技术与多种统计分析方法，定量揭示局地环境条件与区域空间因子在塑造近海水域细菌生物地理格局过程中的相对贡献。