The Process Of Hdac11 Assay Development: Linearity And Km Calculation

With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated. <strong>Note: </strong>1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%). 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.基于此前优化的实验条件，我们获取了组蛋白去乙酰化酶11（HDAC11）的线性检测范围，并计算得到其对应底物的米氏常数（Km）。 注意： 1. 本次测定所用的缓冲液中，牛血清白蛋白（BSA, Bovine Serum Albumin）的浓度为0.5 mg/ml（而非0.5%）。 2. 在7.5 μL的显色液中，还添加了40 μM的曲古抑菌素A（TSA, Trichostatin A）。