GSE42496: RNA sequencing of stripe rust infected wheat leaves and isolated haustoria

Summary: The fungus Puccinia striiformis f.sp. tritici (PST) is the causal pathogen of stripe rust in wheat. New highly virulent PST races appeared at the beginning of this century and spread rapidly causing significant yield losses in wheat production worldwide. Race PST-08/21 was isolated in the UK in 2008 Yr1, Yr2, Yr3, Yr4, Yr6, Yr9, Yr17, Yr27, Yr32, YrRob, YrSol. We applied the RNAseq approach to refine the gene prediction in de novo assembled PST 08/21 contigs and to determine which genes are expressed during wheat infections. Overall Design: Total RNA was extracted from a pool of stripe rust infected wheat leaves and from two biological replicates of haustoria isolates.

摘要：小麦条锈柄锈菌（Puccinia striiformis f.sp. tritici，简称PST）是引发小麦条锈病的致病病原菌。本世纪初出现了新的高毒力PST生理小种，并快速扩散，在全球范围内造成小麦生产的严重产量损失。PST-08/21小种于2008年在英国分离得到，其对应的抗病基因为Yr1、Yr2、Yr3、Yr4、Yr6、Yr9、Yr17、Yr27、Yr32、YrRob、YrSol。本研究采用RNA测序（RNAseq）技术，对从头组装（de novo）的PST 08/21重叠群（contigs）进行基因预测优化，并明确小麦侵染过程中表达的基因。整体实验设计：从条锈菌侵染的小麦叶片混合样本以及两个生物学重复的吸器（haustoria）分离物中提取总RNA。