Phenotypic analysis of resident peritoneal MØ subsets.

SPM and LPM were gated as shown in Figure 1 and the median fluorescence intensity (MFI) of F4/80, CD11b, IAb, CD80, CD86, CD40, LY6C, DC-SIGN, Dectin-1 and TLR4 was analyzed by flow cytometry. All acquisitions were performed using a 5-color staining combination. The value for the expression of each surface marker was obtained by subtracting the MFI of the fluorescence-minus-one control (FMO) from the MFI of the whole stained cell population [23], [25], as described in the Materials and Methods (M&M) section. Data are representative of more than 3 independent experiments.

按照图1所示方式对SPM与LPM进行门控，通过流式细胞术分析F4/80、CD11b、IAb、CD80、CD86、CD40、LY6C、DC-SIGN、Dectin-1及TLR4的中位荧光强度（median fluorescence intensity, MFI）。所有流式数据采集均采用五色染色组合方案。各表面标志物的表达值通过从全染色细胞群的中位荧光强度中减去荧光减一对照（fluorescence-minus-one control, FMO）的中位荧光强度计算得到[23,25]，具体方法详见材料与方法（M&M）部分。本数据为3次以上独立重复实验的代表性结果。