Extracellular spinal recordings: spontaneous activity and fictive locomotion

In vitro extracellular recording dataset of spontaneous activity in normal artificial cerebrospinal fluid (aCSF) and drug-induced fictive locomotion associated with Pocratsky et al 2023, Science Translational Medicine

ENG_spontAct_aCSF.xlsx: raw and analysed data from extracellular recordings from lumbar spinal ventral roots of postnatal spinal cords (postnataly day 3-5). Data reflects spontaneous activity observed when cords were perfused with artifical cerebrospinal fluid (aCSF). Groups: Littermate control and spinal Tor1a d-cko mice that develop early onset generalised torsional dystonia

ENG_fictiveLocomotion.xlsx: raw and analysed data from extracellular recordings from lumbar spinal ventral roots of postnatal spinal cords (postnatal day 1-5). Data reflects stable rhythmic bursting activity observed when cords were perfused with aCSF mixed with a cocktail of NMDA, 5-HT, and dopamine (for p3-p5 recordings, only). 

Full methodological details can be found with publication

本数据集源自Pocratsky等人2023年发表于《Science Translational Medicine》的研究，包含正常人工脑脊液（artificial cerebrospinal fluid, aCSF）环境下的自发活动，以及药物诱导虚拟运动相关的体外细胞外记录数据。

ENG_spontAct_aCSF.xlsx：包含出生后3~5日龄脊髓标本腰骶段腹根的细胞外记录原始数据与分析结果，记录了标本以人工脑脊液（aCSF）灌流时观测到的自发活动。实验分组包括同窝对照小鼠，以及可早发全身性扭转肌张力障碍的脊髓特异性Tor1a条件性敲除（Tor1a d-cko）小鼠。

ENG_fictiveLocomotion.xlsx：包含出生后1~5日龄脊髓标本腰骶段腹根的细胞外记录原始数据与分析结果，记录了标本以含N-甲基-D-天冬氨酸（NMDA）、5-羟色胺（5-HT）与多巴胺的人工脑脊液（aCSF）灌流时观测到的稳定节律爆发活动（该灌流方案仅适用于3~5日龄标本的记录）。

完整的实验方法细节可参阅该发表文献。