Wnt-pathway genetic lesions in MSI colon tumors and cell lines.

NKD1, AXIN2, TCF7L2, CTNNB1, and APC gene mutation status in 40 MSI-CRC tumors (top) and 11 cell lines (bottom). Key: −, no lesion; ins, nucleotide insertion; del, nucleotide deletion; /−, allelic deletion. For NKD1 and AXIN2, mutation in indicated nucleotide is designated, while for TCF7L2 the status of the poly(A) tract (A8–wild-type; A9–mutant) is designated. CTNNB1 exon-3 was screened for activating mutations (M). For APC, tumors positive for protein-truncation (T) are indicated [45]. Presence (+) or absence (−) of APC and CTNNB1 lesions in each cell line is as described [48].

本数据集包含40例微卫星不稳定型结直肠癌（MSI-CRC）肿瘤（上方组）与11株细胞系（下方组）中NKD1、AXIN2、TCF7L2、CTNNB1及APC基因的突变状态。图例说明：− 代表无病变；ins 代表核苷酸插入；del 代表核苷酸缺失；/− 代表等位基因缺失。针对NKD1与AXIN2基因，将标注指定位点的核苷酸突变；对于TCF7L2基因，则标注其多聚腺苷酸（poly(A) tract）序列的状态（A8为野生型，A9为突变型）。针对CTNNB1基因的第3外显子，筛查其激活突变，突变以（M）标注。对于APC基因，存在蛋白截短突变（T）的肿瘤已标注，相关信息引自文献[45]。各细胞系中APC与CTNNB1变异的存在（+）与缺失（−）情况详见文献[48]。