Genome-wide maps of histone modifications in response to CO2 fluctuation in Nannochloropsis oceanica.. Genome-wide maps of histone modifications in response to CO2 fluctuation in Nannochloropsis oceanica.

Epigenetic regulation in response to CO2 fluctuation and epigenome-association with phenotypic plasticity of CCM are firstly uncovered in marine microalga Nannochloropsis oceanica IMET1. The result showed that Kbu and H3K9m2 histone modifications were present in N. oceanica IMET1. Moreover, both Kbu and H3K9m2 positively regulated gene expression. Overall design: Examination of 2 different histone modifications under different CO2 concentrations in Nannochloropsis oceanica cells.

本研究首次在海洋微拟球藻（Nannochloropsis oceanica）IMET1中揭示了响应二氧化碳波动（CO2 fluctuation）的表观遗传调控（Epigenetic regulation），以及碳浓缩机制（CCM）的表型可塑性（phenotypic plasticity）与表观基因组的关联。研究结果表明，海洋微拟球藻IMET1中存在赖氨酸丁酰化（Kbu）与组蛋白H3赖氨酸9二甲基化（H3K9m2）两种组蛋白修饰（histone modifications）。此外，Kbu与H3K9m2均可正向调控基因表达（gene expression）。实验设计概述：检测海洋微拟球藻细胞在不同二氧化碳浓度（CO2 concentrations）下的两种组蛋白修饰水平。