tuf amplicon sequencing for profiling of Bacillus genera

Bacillus species are indistinguishable from standard 16S amplicon sequencing. We developed tuf amplicon-based approach to profile Bacillus species in communities and benchmarked its performance by comparing it with other amplicon approaches.

芽孢杆菌属物种无法通过标准16S扩增子测序进行区分。本研究开发了基于tuf基因的扩增子分析方法，用于解析群落中的芽孢杆菌属物种组成，并通过与其他扩增子方法对比，对该方法的性能开展了基准测试。