Gene expression analysis of mouse ID8 ovarian cancer cells stimulated with CCL6 in vitro. Gene expression analysis of mouse ID8 ovarian cancer cells stimulated with CCL6 in vitro

We used mouse Clariom-S microarrays to study the gene expression profile of ID8 cancer cells stimulated with C-C Chemokine Ligand 6 (CCL6). Overall design: RNA extracted from ID8 mouse ovarian cancer cell lines (n=3) and ID8 cells stimulated with 200ng/mL CCL6 for 6 hours (n=3) was hybridized on Affymetrix Mouse Clariom S microarrays. We sought to identify key regulatory pathways activated in ovarian cancer cells upon stimulation with CCL6 that play a role in migration and metastasis. To understand the signaling mechanism of CCR1-CCL6 interaction in ovarian cancer, we generated biological replicates for unstimulated ID8 cells and ID8 cells stimulated with 200ng/mL CCL6 for 6 hours.

本研究借助小鼠Clariom-S微阵列（Clariom-S microarrays），分析经C-C趋化因子配体6（C-C Chemokine Ligand 6，CCL6）刺激的ID8癌细胞的基因表达谱。实验设计概况：从ID8小鼠卵巢癌细胞系（n=3）以及经200ng/mL CCL6刺激6小时的ID8细胞中提取的RNA，均在Affymetrix小鼠Clariom S微阵列上完成杂交。本研究旨在筛选经CCL6刺激后，卵巢癌细胞中被激活的、参与细胞迁移与转移过程的关键调控通路。为阐明卵巢癌中CCR1-CCL6相互作用（CCR1-CCL6 interaction）的信号传导机制，本研究制备了未刺激ID8细胞与经200ng/mL CCL6刺激6小时的ID8细胞的生物学重复样本。