Genome-wide H3K4me3 after MK2206/DMSO treatment of T47D cells

We perfomed ChIP-seq using H3K4me3 antibodies in T47D cells after 24 hour treatment with the AKT inhibitor MK2206 or DMSO. We demonstrate that at a selected group of loci H3K4me3 is affected by AKT inhibition. Comparison of genome-wide H3K4me3 binding after AKT inhibition vs vehicle

本研究使用H3K4me3抗体（H3K4me3 antibody），在经AKT抑制剂MK2206或二甲基亚砜（Dimethyl Sulfoxide，DMSO）处理24小时的T47D细胞中开展染色质免疫共沉淀测序（Chromatin Immunoprecipitation Sequencing，ChIP-seq）实验。本研究证实，在选定的一组基因座（locus，复数形式为loci）上，H3K4me3的修饰水平会受到AKT抑制的影响。本数据集包含AKT抑制剂处理组与溶剂对照（vehicle）组的全基因组H3K4me3结合情况比较分析。