Deciphering cis-regulatory logic with 100 million synthetic promoters (MNase)

In order to test our model of gene regulation, we measured nucleosome occupancy of our random promoter library using Mnase-seq. The pTpA library was grown in glucose (YPD), with four different replicates.

为验证本研究的基因调控模型，我们采用微球菌核酸酶测序（Mnase-seq）技术测定了随机启动子文库的核小体占据率。本次实验中的pTpA文库于葡萄糖（YPD）培养基中培养，共设置四次生物学重复。