BRD4 Chromatin immunoprecipitation and Sequencing
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
We report BET bromodomain inhibitors (BETi) as regulators of keratinocyte stem cell fate. BETi displace BET proteins, including BRD4 from chromatin, inducing acute transcriptional changes at selected target genes. To gain insight into the compound mechanism of action, we assayed BRD4 chromatin occupancy genome-wide in keratinocytes subjected to BETi treatment. Specifically, BRD4 chromatin binding was analyzed following two treatments [125nM (low) versus 1250nM (high)] and three time points (30min, 6h and 6h treatment followed by compound removal for 3h (6m3h)).
本研究报道BET溴结构域抑制剂（BET bromodomain inhibitors, BETi）可作为角质形成细胞干细胞命运的调控因子。BETi可将包括BRD4在内的BET家族蛋白从染色质上解离下来，进而在特定靶基因处诱导快速的转录变化。为深入探究该化合物的作用机制，我们对经BETi处理的角质形成细胞开展了全基因组范围的BRD4染色质结合占有率检测。具体而言，我们针对两种处理浓度[125nM（低剂量组）与1250nM（高剂量组）]以及三个时间点（30分钟、6小时，以及先经6小时处理后移除化合物并孵育3小时（记作6m3h）），分别分析了BRD4的染色质结合情况。
提供机构：
Novartis Institutes for Biomedical Research
创建时间：
2022-02-20
