Stem Cell transcriptional response to Microglia-Conditioned Media

This paper demonstrates that whereas SOX2+ cells in HDB are highly susceptible to ZKV, SOX2+ primary GBM samples exhibit moderate to high resistance to infection. Expression profiling revealed an innate immune signature due to microglia infiltration in highly resistant GBM samples that was lacking in HDB. This data was generated to proflile the transcriptional response to microglia-secreted factors in stem cells from glioma cell lines and human developing brain. We find that Interferon-stimulated genes are the main targets upregulated in response to microglia conditioned media exposure. Overall design: GCGR glioma stem cell lines (G322, G334) and primary human developing brain cultures (BRC2251) were exposed to microglia-conditioned media (067,069,070) or to GSC-conditioned control media (G322, G334, BRC2251) media

本研究证实，人脑发育组织（human developing brain, HDB）内的SOX2阳性（SOX2+）细胞对ZKV高度易感，而SOX2阳性原发性胶质母细胞瘤（Glioblastoma Multiforme, GBM）样本则表现出中度至重度的感染抵抗性。表达谱分析显示，抵抗性较强的GBM样本中因小胶质细胞（microglia）浸润呈现出先天免疫特征，而该特征在HDB样本中并未观测到。本数据集旨在分析胶质瘤细胞系及人脑发育组织来源干细胞对小胶质细胞分泌因子的转录应答特征。研究发现，干扰素刺激基因（Interferon-stimulated genes）是暴露于小胶质细胞条件培养基后上调表达的核心靶标。整体实验设计：将GCGR胶质瘤干细胞系（G322、G334）及原代人脑发育组织培养物（BRC2251）分别暴露于小胶质细胞条件培养基（067、069、070），或胶质瘤干细胞（Glioma Stem Cell, GSC）条件对照培养基（G322、G334、BRC2251）。