Transcriptional analysis of IGF1 treatment of mouse tenocytes over a 24 hour time course. Transcriptional analysis of IGF1 treatment of mouse tenocytes over a 24 hour time course

We report RNA sequencing data from tenocytes treated with IGF1. Tenocytes were obtained from the tail tendons of adult C57Bl/6 mice via collagenase digestion. Tenocytes were grown to 60% confluence, and then treated with 100ng/mL of recombinant IGF1 for a period of 0, 1, 2, 6, or 24 hours. Experiments were conducted in quadruplicate. RNA was isolated and prepared for RNA sequencing. Overall design: Differential expression of mRNAs were evaluated from tenocytes isolated from tail tendons of adult wild type C57Bl/6 mice that were treated with recombinant IGF1 for 0, 1, 2, 6, and 24 hours.

本研究报道了经胰岛素样生长因子1（Insulin-like Growth Factor 1, IGF1）处理的肌腱细胞（tenocytes）的RNA测序数据。肌腱细胞通过胶原酶消化法从成年C57Bl/6小鼠的尾肌腱中分离获得。将肌腱细胞培养至60%汇合度后，以100ng/mL的重组胰岛素样生长因子1处理0、1、2、6或24小时，所有实验均设置四次生物学重复。提取各组细胞总RNA并制备RNA测序文库。整体实验设计：本研究以从成年野生型C57Bl/6小鼠尾肌腱中分离的肌腱细胞为材料，分别经重组胰岛素样生长因子1处理0、1、2、6及24小时，以此评估信使RNA（messenger RNA, mRNA）的差异表达水平。