Single-cell RNA sequencing of human various osteosarcoma tissues

We performed the scRNA-seq analysis of 2 primary osteosarcoma tissues ,2 osteosarcoma lymphoid tissues, 4 adjacent tissues of osteosarcoma based on the 10X Genomics platform. Overall design: Primary osteosarcoma tissues,osteosarcoma lymphoid tissues and adjacent tissues of osteosarcoma from 4 patients were used for single cell RNA sequencing by 10X Genomics本研究基于10X Genomics平台，对2例原发性骨肉瘤组织、2例骨肉瘤淋巴组织及4例骨肉瘤旁组织开展了单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）分析。实验整体设计：选取4名患者的原发性骨肉瘤组织、骨肉瘤淋巴组织及骨肉瘤旁组织，通过10X Genomics平台完成单细胞RNA测序。