Effects of CDK8/19 Mediator Kinase inhibition by Senexin B and SNX631 on androgen-regulated gene expression in LNCaP cells. Effects of CDK8/19 Mediator Kinase inhibition by Senexin B and SNX631 on androgen-regulated gene expression in LNCaP cells

RNA-Seq analysis was carried out to investigate the effects of selective CDK8/19 inhibitor Senexin B and SNX631 on gene expression in LNCaP prostate cancer cells treated with or without androgen. Overall design: Androgen-deprived LNCaP cells were treated with vehicle control (0.1% DMSO), CDK8/19 inhibitors (1 μM Senexin B or 500 nM SNX631), synthetic androgen R1881 (0.1 or 1 nM), or a combination of CDK8/19 inhibitior and R1881 for 24 or 72 hours. Each treatment condition was done in biological triplicate or quadruplicate.

本研究通过RNA测序（RNA-Seq）分析，探究选择性CDK8/19抑制剂Senexin B与SNX631对经雄激素处理或未处理的LNCaP前列腺癌细胞的基因表达影响。
实验总体设计：将经去雄激素培养的LNCaP前列腺癌细胞分为多组，分别以溶剂对照（0.1%二甲基亚砜，DMSO）、CDK8/19抑制剂（1μM Senexin B或500nM SNX631）、合成雄激素R1881（0.1或1nM），以及CDK8/19抑制剂与R1881的联合处理组进行处理，处理时长为24或72小时。每种处理条件均设置3次生物学重复或4次生物学重复。