Identification of Sec23ip, part of 14-3-3γ protein network, as a regulator of acute steroidogenesis in MA-10 Leydig cells

<b>Supplemental Figure 1</b>. Image obtained from protein atlas databases. Sections of a testes obtained from a patient were stained with Sec23ip antibody. Patient data and cell types with Sec23ip expression are indicated. <br> <b>Supplemental Table 1</b>. A list of proteins from the 14-3-3γ interactome in cAMP-treated MA-10 cells at times 0, 30, 60 and 120. The proteins are listed based on progressive increase in interactions with 14-3-3γ. <b>Supplemental Table 2</b>. A selected list of 14-3-3γ interactome containing proteins that showed consistent interactions at all time points across n= 3. <br> <b>Supplemental Table 3</b>. Relative gene expression for Sec23ip in steroidogenic tissues in human and mice. This data was obtained from biogps database using the probeset 209175-at for human and probeset 1433627-at for mice. <b>Supplemental Table 4</b>. The sequences of 3 siRNA used for Sec23ip knock down. <b></b>

**补充图1**：图像取自蛋白质图谱（Protein Atlas）数据库。一名患者的睾丸组织切片经Sec23ip抗体染色，图中标注了患者相关信息以及表达Sec23ip的细胞类型。<br>**补充表1**：本列表收录了经环腺苷酸（cAMP）处理的MA-10细胞在0、30、60和120分钟时的14-3-3γ互作组蛋白，蛋白按与14-3-3γ的互作强度逐步递增排序。<br>**补充表2**：本精选列表收录了14-3-3γ互作组蛋白，包含在3次生物学重复（n=3）的所有时间点均呈现稳定互作的蛋白。<br>**补充表3**：本表格收录了人和小鼠类固醇生成组织中Sec23ip的相对基因表达量数据，该数据取自BioGPS数据库，人类样本使用探针组209175-at，小鼠样本使用探针组1433627-at。<br>**补充表4**：本表格收录了用于Sec23ip基因敲低的3条小干扰RNA（siRNA）的序列。<br>