P&G gingivitis Targeted Locus

Gingivitis is the most common gum infection, yet its etiology remains elusive. We designed a retrogression-progression model (RPM) to simulate the development and reoccurrence of gingivitis. Fifty adults underwent a controlled transition from naturally occurring gingivitis (NG) to healthy gingivae (Baseline), then back to experimental gingivitis (EG). Within-subject temporal dynamics in taxonomic structure (for the 150 plaque microbiota samples) and functional profiles (for 18 of them) were observed via 16S rRNA gene and shotgun metagenomic sequencing respectively. 16S barcoded pyrosequencing for 273 oral plaque samples.

牙龈炎（Gingivitis）是最为常见的牙龈感染性疾病，但其病因学机制仍尚不明确。我们构建了倒退-进展模型（RPM）以模拟牙龈炎的发生发展与复发过程。招募50名成年受试者，通过可控干预使其从自然发生的牙龈炎（NG）状态过渡至健康牙龈状态（基线期），随后再次诱导为实验性牙龈炎（EG）。针对150份菌斑微生物组样本，我们通过16S核糖体RNA基因（16S rRNA gene）测序解析了受试者内部的分类学结构时序动态；针对其中18份样本，则采用鸟枪宏基因组测序（shotgun metagenomic sequencing）分析其功能谱特征。此外，本数据集还包含273份口腔菌斑样本的16S条形码焦磷酸测序（16S barcoded pyrosequencing）数据。