Gene expression profile at single nuclei level of cells from mouse hippocampus

We performed single-nucleus RNA-seq and chromatin accessibility analyses (snRNA-seq and ATAC-seq) in the hippocampus of wild-type (WT) mice and mice carrying amyloid precursor protein knock-in mutations (APPNL-G-F) in response to chronic wheel-running exercise. 8-week old female WT and APPNL-G-F mice were put into cages with running wheel for voluntary exercise for 10 weeks. Mice from 4 different conditions (WT_RT, WT_EX, APP_RT, APP_EX) were subjected to single nuclei RNA and ATAC sequencing.

本研究针对慢性转轮运动干预，对野生型（Wild-Type，WT）小鼠及携带淀粉样前体蛋白敲入突变（APP^NL-G-F）的小鼠的海马组织，开展了单细胞核RNA测序（single-nucleus RNA-seq，snRNA-seq）与染色质可及性测序分析（Assay for Transposase-Accessible Chromatin using sequencing，ATAC-seq）。研究选取8周龄的雌性WT及APP^NL-G-F小鼠，将运动组小鼠置于配备跑轮的饲养笼中进行为期10周的自愿转轮运动，静止组小鼠则饲养于普通笼具中；最终对野生型静止组（WT_RT）、野生型运动组（WT_EX）、APP突变静止组（APP_RT）及APP突变运动组（APP_EX）共4组不同处理条件的小鼠完成了单细胞核RNA测序与ATAC测序。