CTL differentiation: WT vs. IL-2Ra deficient CTL. Mus musculus

Gene expression analysis of WT and IL-2Ra-deficient CTL (P14) isolated 8 days after inffection with LCMV. The goals of the study are to assess the impact of IL-2 signals on effector and memory CTL differentiation. Overall design: This is a 2-condition experiement comparing WT P14 Tg T cells eight days after LCMV infection to IL-2Ra-deficient P14 Tg T cells isolated at the same time from the same host. Cells are isolated via FACS-sorting form the spleen.

本数据集针对淋巴细胞脉络丛脑膜炎病毒（Lymphocytic Choriomeningitis Virus, LCMV）感染8天后分离的野生型（Wild Type, WT）与IL-2Ra缺陷型（IL-2Ra-deficient）P14细胞毒性T淋巴细胞（Cytotoxic T Lymphocyte, CTL）开展基因表达分析。本研究旨在评估IL-2信号对效应性与记忆性细胞毒性T淋巴细胞分化的影响。整体实验设计：本实验为双条件对照实验，对比LCMV感染8天后分离的野生型P14转基因T细胞，与同期从同一宿主中分离的IL-2Ra缺陷型P14转基因T细胞。细胞通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）从脾脏中分离得到。