Figure 8: Raw data, MATLAB scripts, and processed videos of lightsheet image stacks of GUVs

3D volume stacks of images of GUVs embedded in agarose. GUVs made of DPPC or DOPC were labelled with di-4-ANEPPDHQ. The fluorescence lifetime of the di-4-ANEPPDHQ changes with the membrane lipid packing state and thus the bilayer constituent.The MATLAB script <b>makeFLIM.m</b> produces videos and graphs from the SPAD output data analyzed in TRI2. The calibration data from the SPADlinearization package (https://doi.org/10.18742/21325584) will be needed to run this script.This dataset is a supplement to the article "A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos"

本数据集包含包埋于琼脂糖中的巨型单层囊泡（Giant Unilamellar Vesicles, GUVs）的三维容积图像栈。由二棕榈酰磷脂酰胆碱（DPPC）或二油酰磷脂酰胆碱（DOPC）制备的GUVs经荧光探针di-4-ANEPPDHQ标记。di-4-ANEPPDHQ的荧光寿命会随膜脂堆积状态以及双层膜的组成成分发生改变。MATLAB脚本makeFLIM.m可从经TRI2分析的单光子雪崩二极管（Single-Photon Avalanche Diode, SPAD）输出数据中生成视频与图表。运行该脚本需使用SPAD线性化软件包的校准数据，该数据的DOI链接为https://doi.org/10.18742/21325584。本数据集是论文《用于光片荧光寿命成像（fluorescence lifetime imaging microscopy, FLIM）及FLIM视频的定制化数据处理算法与时域单光子计数SPAD阵列相机》的补充数据集。