Round 1 RNA-sequencing analysis of the OsIMA overexpression and knockdown rice lines and Fe deficiency responsiveness of non-transformant (NT) rice.

We report the comparison of transcript expression of Fe-sufficient roots and leaves of OsIMA overexpression lines IMA1OX-7, 9, IMA2OX-2, 5 and corresponding NTby RNA-Seq. Total RNA was extracted from Fe-sufficient roots and leaves of IMA1OX-7, 9, IMA2OX-2, 5 and corresponding NT, and used for RNA-Seq library preparation, sequencing, mapping and quantification of each gene expression as read per million (RPM).

我们通过RNA测序（RNA-Seq）对OsIMA过表达株系IMA1OX-7、IMA1OX-9、IMA2OX-2、IMA2OX-5及其对应的非转化对照（NT）在铁充足条件下的根系与叶片转录本表达情况进行了比较分析。实验中，我们从上述各株系及对应NT的铁充足条件下的根系和叶片中提取总RNA，用于RNA-Seq文库构建、测序、序列比对，以及以每百万读段数（RPM，reads per million）为量化单位的各基因表达量定量分析。