The effect of serum on the HIV enhancing of defensins.

Pseudotyped HIV-1JR-FL luciferase reporter virus was incubated with or without defensins (5.6 µM) in the presence of FBS or human serum (HS) at various concentrations for 1 h. FBS or HS at a final concentration of 10% was added to the virus-defensin mixture before exposure to HeLa-CD4-CCR5. Luciferase activity was determined 48 h after infection. Results are expressed as fold increase compared to samples with the same concentration of FBS or HS in the absence of defensins. Assays were performed on triplicate cultures; results represent two independent experiments (mean ± SD).将假型HIV-1JR-FL荧光素酶报告病毒（Pseudotyped HIV-1JR-FL luciferase reporter virus）与浓度为5.6 μM的防御素（defensins）共孵育，或不添加防御素，在不同浓度的胎牛血清（Fetal Bovine Serum, FBS）或人血清（Human Serum, HS）存在的条件下孵育1小时；随后在将该病毒-防御素混合物暴露于HeLa-CD4-CCR5细胞之前，向其中加入终浓度为10%的FBS或HS。于感染后48小时检测荧光素酶活性。实验结果以与不含防御素且FBS或HS浓度相同的对照组样品相比的增幅倍数表示。所有实验均设置三次重复培养，结果来自两次独立重复实验，以平均值±标准差（SD）呈现。