Zea mays cultivar:Mo17 Transcriptome or Gene expression. Zea mays cultivar:Mo17

Bipolaris zeicola is a fungal pathogen that causes Northern corn leaf spot (NCLS), which is a serious foliar disease in maize and one of the most significant pathogens affecting global food security. Here, we report genome wide transcriptional profile analysis of maize leaf development inoculation with B.zeicola using next generation sequencing (NGS). We performed High-Throughput Digital Gene Expression analysis identify differential gene expression in resistant inbred lines Mo17 infection with B.zeicola, compared with CK (mock-treat). Deep sequencing was subsequently used to compare the digital gene expression (DGE) profiles of the healthy plants with the infected plants at four successive disease development stages, including CP (Contact period), PP (penetration period), IP (incubation period), PP (disease period). Of which, 466 differentially expressed genes were identified in all these libraries, KEGG pathway analysis of these genes suggested that involved in many biological processes of systemic symptom development, such as Plant hormone signal transduction, Starch and sucrose metabolism, Phenylpropanoid biosynthesis, and Photosynthesis. Our systematic analysis provides comprehensive transcriptomic information regarding systemic symptom development in fungal-infected plants. This information will help further our understanding of the detailed mechanisms of plant responses to fungal infection.

平脐蠕孢菌（Bipolaris zeicola, B.zeicola）是一种可引发玉米北方叶斑病（Northern corn leaf spot, NCLS）的真菌病原菌，该病害是玉米上的严重叶部病害，也是影响全球粮食安全的最重要病原菌之一。本研究采用下一代测序（next generation sequencing, NGS）技术，对接种平脐蠕孢菌的玉米叶片开展全基因组转录谱分析。我们通过高通量数字基因表达（High-Throughput Digital Gene Expression）分析，对比了接种平脐蠕孢菌的抗病自交系Mo17与空白对照（mock-treat, CK）样本间的差异基因表达情况。随后，我们采用深度测序技术，对比了健康植株与处于四个连续病害发育阶段的接种植株的数字基因表达（DGE）谱，这四个阶段分别为接触期（Contact period, CP）、侵入期（penetration period, PP）、潜育期（incubation period, IP）以及显症期（disease period, PP）。在所有测序文库中共鉴定出466个差异表达基因；对这些基因进行KEGG通路富集分析发现，它们参与了系统症状发育的诸多生物学过程，包括植物激素信号转导、淀粉与蔗糖代谢、苯丙烷类生物合成以及光合作用。本系统性分析为真菌侵染植株的系统症状发育过程提供了全面的转录组学信息，该成果将有助于我们进一步解析植物响应真菌侵染的具体分子机制。