Genome-wide characterization of long nonpolyadenylated RNAs

We have used deep sequencing to explore the repertoire of both poly(A)+ and poly(A)- RNAs from two standard cell lines, HeLa cells and human embryonic stem cell (hESC) H9 cells. Overall design: Examination of nonpolyadenylated and polyadenylated RNA in 2 cell types.本研究采用深度测序技术，对两种标准细胞系——海拉（HeLa）细胞与人类胚胎干细胞（hESC）H9细胞中的聚腺苷酸化（poly(A)+）RNA及非聚腺苷酸化（poly(A)-）RNA的全套转录本谱进行了探究。实验设计概述：针对两种细胞类型中的非聚腺苷酸化RNA与聚腺苷酸化RNA展开检测分析。