Gene expression profiles in GC B cells during acute and chronic viral infection. Gene expression profiles in GC B cells during acute and chronic viral infection

To study how chronic viral infection disrupts B cell differentiation pathways and identify key intrinsic regulators in an endogenous polyclonal response, we used the comparative LCMV model. Wild-type mice were infected with either the acute (WE) versus chronic (Docile) strains of LCMV, GC B cells (CD19+IgDloCD95+CD38loCD138-) isolated 14 days post-infection, and RNA-seq was undertaken Overall design: 4 samples . Each group has at least 2 biological replicates.

为探究慢性病毒感染如何破坏B细胞分化通路，并在内源性多克隆应答中鉴定关键内在调控因子，我们采用了比较性淋巴细胞脉络丛脑膜炎病毒（Lymphocytic Choriomeningitis Virus, LCMV）模型。将野生型小鼠分别感染急性型（WE株）与慢性型（Docile株）LCMV毒株，于感染后14天分离得到生发中心（Germinal Center, GC）B细胞（CD19+IgDloCD95+CD38loCD138-），随后开展RNA测序（RNA-seq）实验。实验整体设计：共包含4个样本，每组至少设置2次生物学重复。