Comparison of gene expression in macrophages tolerised by different toll-like receptor ligands. Mus musculus

To investigate the similarity of toll-like receptor tolerance in macrophages stimulated with different toll-like receptor ligands we stimulated naïve or tolerant macrophages with ligands for TLR4, TLR2, TLR3 and TLR9. The data identifies a core set of genes that are tolerised by all ligands and genes that show TLR specific patterns. Overall design: Bone marrow derived macrophages were stimulated with four different toll-like receptor ligands; LPS, CpG, Pam3csk4 and PolyI:C. In addition some cells received a pre-treatment with the same ligand prior to restimulation to induce a tolerant state. Gene expression was measured at 4 hours post stimulation with each ligand. Three independent experiments were performed to measure gene expression changes between each macrophage group. Of the 27 samples, LPSTolerance_3 and Pam3csk4_2 were excluded from the analysis entirely due to low sample quality – they are included in this dataset for completeness only.

为探究经不同 toll 样受体（toll-like receptor, TLR）配体刺激的巨噬细胞中 TLR 耐受的相似性，我们使用 TLR4、TLR2、TLR3 及 TLR9 的配体，分别刺激未致敏（naïve）或已耐受的巨噬细胞。本数据集鉴定出一组可被所有配体诱导耐受的核心基因，以及呈现 TLR 特异性表达模式的基因。实验整体设计：采用四种不同的 TLR 配体——脂多糖（lipopolysaccharide, LPS）、CpG、Pam3csk4 及聚肌胞苷酸（PolyI:C）——刺激骨髓来源巨噬细胞。部分细胞在再次刺激前，先用同种配体进行预处理以诱导耐受状态。于每次配体刺激后4小时检测基因表达水平。我们开展了三次独立实验，以测定各巨噬细胞组间的基因表达变化。在27份样本中，LPSTolerance_3 与 Pam3csk4_2 因样本质量过低被完全排除在分析之外，仅为保证数据集完整性而将其纳入本数据集。