Histological scoring system of burned dermis.

The present study aimed to investigate the efficacy of different doses of Eucerin-based ointment containing opium compared to routine treatment on experimentally induced burn injury. Male Wistar rats were divided into five experimental groups for topical application: silver sulfadiazine 1% (SSD), Eucerin-based ointment containing opium at concentrations of 0.05%, 0.5%, and 5%, and a Eucerin-based ointment containing 0.05% opium plus SSD (mixed group), following burn wound induction using the comb burn model. An equal volume of different ointments was administered topically. The wound appearances of different groups were photographed at different time points for 21 days. In addition, blood and skin samples were taken 2 and 10 days after thermal injury to assess inflammatory parameters and oxidative stress markers. Also, the liver enzyme activity and kidney function tests were assessed on day 10. The body weight in Opium 5% significantly decreased compared to all other groups after ten days. The wound area was significantly reduced (P0.05). The serum concentrations of interleukin-10 in the mixed group were significantly higher compared to the SSD group on day 10. After ten days, groups were not different regarding AST, ALT, and creatinine concentrations (p>0.05). Histopathological analysis revealed that the wound healing efficacy of opium at its lowest concentration (0.05%) surpassed that of silver sulfadiazine (SSD). Furthermore, the combination of 0.05% opium with SSD enhanced the wound repair potential of SSD in burn injuries. This study showed that opium ointment delayed wound closure dose-dependently. Low doses of topical opium ointment and its mixed application with silver sulfadiazine had a protective role in thermal-induced injury.本研究旨在探究不同剂量含鸦片的优色林（Eucerin）软膏，相较于常规治疗方案，对实验诱导烧伤创面的治疗效果。选用雄性Wistar大鼠，采用梳状烧伤造模法诱导烧伤后，将其分为5组进行局部给药：1%磺胺嘧啶银（silver sulfadiazine，SSD）组、浓度分别为0.05%、0.5%及5%的含鸦片优色林软膏组，以及0.05%鸦片优色林软膏联合SSD的混合给药组。各组均给予等量软膏进行局部涂抹。在造模后21天内的不同时间点，对各组创面外观进行拍照记录。此外，分别于热损伤后第2天和第10天采集血液与皮肤样本，以检测炎症指标与氧化应激标志物；并于第10天检测肝酶活性与肾功能指标。给药10天后，5%鸦片组大鼠体重较其余所有组别显著降低。各组创面面积均显著缩小（P0.05）。第10天时，混合给药组的白细胞介素-10（interleukin-10）血清浓度显著高于SSD组。给药10天后，各组天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）及肌酐浓度均无显著差异（p>0.05）。组织病理学分析显示，最低浓度（0.05%）的鸦片软膏，其创面愈合效果优于磺胺嘧啶银（SSD）。此外，0.05%鸦片软膏与SSD联合使用，可增强SSD对烧伤创面的修复能力。本研究表明，鸦片软膏可呈剂量依赖性延缓创面闭合。局部应用低剂量鸦片软膏，或将其与磺胺嘧啶银联合使用，对热损伤具有保护作用。