Transcriptome profiling of OsbZIP48 rice transgenics

Rice transgenic plants of the bZIP encoding gene, OsbZIP48, in the Pusa Basmati 1 (PB1) variety have been found to display differences in the total height. In order to elucidate changes at the transcriptome level, microarray of the 10-day-old seedlings of over-expresseion (OE) and knock-down (KD) lines along with vector control (VC) were carried out. Seedlings of OsbZIP48-OE, OsbZIP48-KD and VC grown for seven days at 28±1oC in light. RNA from the whole seedlings were extracted from each sample and hybridized on rice Affymetrix microarrays. Two biological replicates of each sample were used for microarray analysis. Overall, ten samples were analyzed representing the transgenic lines and vector control constructs.

本研究发现，Pusa Basmati 1（PB1）品种中转入碱性亮氨酸拉链（bZIP）编码基因OsbZIP48的转基因水稻植株，其总株高存在差异。为阐明该基因在转录组层面的调控变化，本实验对10日龄的过表达（OE）、敲低（KD）株系及空载对照（VC）的幼苗开展了基因芯片分析。OsbZIP48过表达株系、OsbZIP48敲低株系与空载对照的幼苗均于28±1℃光照条件下培养7天。提取各样本全株幼苗的总RNA，将其与水稻Affymetrix基因芯片进行杂交。每个样本设置2次生物学重复用于芯片分析。本次分析共纳入10个样本，分别对应各转基因株系与空载对照构建体。