Effect of use of injected trace minerals (copper, zinc, selenium, and manganese) and vitamins (A and E) on oxidative stress and neutrophil function in females of Hostein cows during transitionl

ABSTRACT This study evaluates the metaphylactic of the subcutaneous administration of a trace minerals and vitamins A and E, on the oxidative stress and neutrophil function in Holsteins cows under the transition period. Sixty females were divided in two groups: group with trace minerals and vitamins (GMTV) (n= 30) and group control (GC) (n= 30). There was no significant difference between those groups; however, we find significant difference between treatment and day for Superoxide dismutase (SOD) values with higher activity of this enzyme in females GMTV on the weeks next to the parturition. Still, there was difference on day and, for interactions between treatment and day for TBARS, were females GMTV showed lower values of TBARS in all moments, except on day seven after the parturition. For leucocytes, there is a significant effect by day, interaction on treatment and day on neutrophils, and interaction treatment and day for neutrophil phagocytosis, were females GMTV showed lower values of leucocytes and neutrophils next to the parturition, and an increase of neutrophils phagocytosis. In summary, cows from the GMTV group had an improvement on the immune and oxidative systems, probably correlated with the administration of this supplement.

摘要 本研究评估了皮下注射微量矿物质与维生素A、E的预防性给药方案，对围产期荷斯坦奶牛（Holstein）的氧化应激与中性粒细胞功能的影响。研究将60头泌乳母牛分为两组：微量矿物质与维生素组（GMTV，n=30）与对照组（GC，n=30）。两组初始无显著差异；但研究发现，超氧化物歧化酶（Superoxide dismutase, SOD）活性在处理方式与采样日龄上存在显著交互效应：在分娩前后的数周内，GMTV组母牛的该酶活性更高。此外，硫代巴比妥酸反应物（TBARS）的检测结果存在采样日龄主效应，且处理方式与采样日龄存在交互效应：除分娩后第7天外，GMTV组母牛的TBARS值在所有采样时点均更低。针对白细胞指标，采样日龄存在显著主效应；中性粒细胞计数存在处理方式与采样日龄的交互效应；中性粒细胞吞噬功能同样存在上述交互效应：GMTV组母牛在分娩前后的白细胞与中性粒细胞计数更低，而其中性粒细胞吞噬功能则有所提升。综上，GMTV组奶牛的免疫与氧化调控系统功能均得到改善，推测与该补充剂的施用存在相关性。