miRNA sequencing detecting miRNA cargo from PRP derived exosomes isolated from young donors and old donors

We detected and compared the miRNAs contained in Y-PRP-exos or O-PRP-exos. The results showed that most miRNAs (38) can be identified in both Y-PRP-exos and O-PRP-exos. In addition, hsa-let-7f-5p, hsa-miR-16-5p and hsa-miR-126-3p were top 3 enriched miRNAs in PRP-exos of all donors. These results indicated the heterogeneity of miRNAs in PRP-exos was small, regardless of age. However, the abundance of miRNAs in Y-PRP-exos and O-PRP-exos was quite different, especially in high-ranking miRNAs. The expression of hsa-miR-16-5p and has-let-7f-5p in O-PRP-exos in two old donors was only approximately 1/4-1/2 of that in Y-PRP-exos in two young donors Overall design: To investigate the miRNAs in PRP-exosomes that could regulate adipogenesis and proliferation of Pdgfra+ progenitors and fatty infiltration in muscle injury, we isolated PRP exosomes derived from two young donors and two old donors, respectively, and compared the enriched miRNAs.

本研究检测并比较了年轻富血小板血浆来源外泌体（Young Platelet-Rich Plasma-derived exosomes, Y-PRP-exos）与老年富血小板血浆来源外泌体（Old Platelet-Rich Plasma-derived exosomes, O-PRP-exos）所包含的微小RNA（microRNAs, miRNAs）。结果显示，共38种微小RNA可在两类外泌体中同时被检出。此外，所有供体的富血小板血浆外泌体中，富集程度排名前三的微小RNA为hsa-let-7f-5p、hsa-miR-16-5p与hsa-miR-126-3p。上述结果表明，无论供体年龄如何，富血小板血浆外泌体中微小RNA的异质性均较低。但Y-PRP-exos与O-PRP-exos中微小RNA的表达丰度存在显著差异，在高排名的微小RNA中这一差异尤为突出。两名老年供体的O-PRP-exos中，hsa-miR-16-5p与hsa-let-7f-5p的表达量仅约为两名年轻供体Y-PRP-exos中对应表达量的1/4至1/2。实验整体设计：为探究富血小板血浆外泌体中可调节脂肪生成、血小板衍生生长因子受体α阳性（Platelet-Derived Growth Factor Receptor Alpha Positive, Pdgfra+）祖细胞增殖以及肌肉损伤后脂肪浸润的微小RNA，本研究分别分离了两名年轻供体与两名老年供体来源的富血小板血浆外泌体，并对其中富集的微小RNA进行了比较分析。