Penicillium sp. CF05 Genome sequencing and assembly. Penicillium sp. CF05

Draft genome sequence of Penicillium sp. CF05 (Penicillium section Chrysogena): insights for safety assessment and biotechnological application.The species belonging to the genus Penicillium (Ascomycota) produce a wide range of enzymes and metabolites of great biotechnological interest. Penicillium sp. CF05 was isolated from a plastic waste recycling plant (Latium region, central Italy). Our phylogenetic analysis based on DNA sequences of the ribosomal DNA regions and protein coding genes (including the beta-tubulin and calmodulin genes) revealed that Penicillium sp. CF05 is a taxon of section Chrysogena and is related to Penicillium chrysogenum sensu lato, which are biotechnologically important taxa in the production of the antibiotic penicillin.In this study, the genome of Penicillium sp. CF05 (Penicillium section Chrysogena) was sequenced using an Illumina MiSeq (2x300 bp paired-end reads strategy). Reads were assembled in scaffolds, which represent the draft genome of Penicillium sp. CF05. A comparative genomic analysis of Penicillium sp. CF05 and other Penicillium species (including the penicillin-producing Penicillium species of section Chrysogena) allows us to identify important genes for safety assessment and biotechnological application.The study was funded with the Research Project: “Microbial genomes: biotechnology and safety; INAIL-PAR 2016-2018”.

青霉属未定种CF05（Penicillium sp. CF05，青霉属Chrysogena组，Penicillium section Chrysogena）的草图基因组序列：安全评估与生物技术应用研究洞察。隶属于青霉属（Penicillium，子囊菌门Ascomycota）的物种可产生大量具有重要生物技术价值的酶类与代谢产物。青霉属未定种CF05分离自意大利中部拉齐奥大区（Latium region）的一处塑料废弃物回收厂。本研究基于核糖体DNA区域及蛋白编码基因（包含β微管蛋白基因beta-tubulin与钙调蛋白基因calmodulin）的DNA序列开展系统发育分析，结果显示青霉属未定种CF05属于Chrysogena组类群，与广义产黄青霉（Penicillium chrysogenum sensu lato）亲缘关系相近；后者是生产抗生素青霉素（penicillin）的重要生物技术相关分类群。本研究采用Illumina MiSeq测序平台，以2×300 bp双端读长策略对青霉属未定种CF05的基因组进行测序。测序得到的读段经组装获得支架序列，即该菌株的草图基因组。通过对青霉属未定种CF05与其他青霉属物种（包括Chrysogena组产青霉素青霉物种）开展比较基因组分析，可鉴定出与安全评估及生物技术应用相关的关键基因。本研究得到科研项目"Microbial genomes: biotechnology and safety; INAIL-PAR 2016-2018"的资助。