Next Generation Sequencing Analysis of Wild Type(WT) and Gdf9-Cre;Kat8 fl/fl(Kat8 Gdf9 cKO) Oocyte Transcriptome

Purpose: The goal of this study is to compare oocyte transcriptome profiling in WT and Kat8 Gdf9 cKO mice Methods: Oocyte mRNA profiles of 2 weeks old WT and Kat8 Gdf9 cKO mice were generated by deep sequencing using Illumina HiSeq 2500. Results: Using an optimized data analysis workflow, we mapped about 54-63 million sequence reads per sample to the mouse genome (build mm10) and identified 18,468 transcripts in the oocytes of WT and Kat8 Gdf9 cKO mice. Approximately 5% of the transcripts showed differential expression between the WT and Kat8 Gdf9 cKO oocyte, with a fold change ≥1.5 and p value <0.05. Conclusions: Our study represents the first detailed analysis of oocyte transcriptomes after kat8 deletion, which is generated by RNA-seq technology.Our results show that Kat8 affects numerous gene expression in mouse oocytes. Oocyte mRNA profiles of 2 weeks old WT and Kat8 Gdf9 cKO mice were generated by deep sequencing using Illumina HiSeq 2500.

研究目的：本研究旨在对比野生型（Wild Type，简称WT）与Kat8 Gdf9条件性敲除（Kat8 Gdf9 cKO）小鼠的卵母细胞转录组谱。
研究方法：采用Illumina HiSeq 2500平台进行深度测序，获取2周龄野生型与Kat8 Gdf9条件性敲除小鼠的卵母细胞mRNA表达谱。
研究结果：通过优化后的数据分析流程，我们将每个样本约5400万至6300万条序列读数比对至小鼠基因组（版本mm10），并在两组小鼠的卵母细胞中鉴定出18468个转录本。约5%的转录本在两组间呈现差异表达，其折叠变化≥1.5且p值<0.05。
研究结论：本研究为首次针对Kat8缺失后的小鼠卵母细胞转录组开展的详尽分析，该分析采用RNA测序技术完成。研究结果显示，Kat8可调控小鼠卵母细胞中众多基因的表达。
本研究通过Illumina HiSeq 2500平台进行深度测序，获取了2周龄野生型与Kat8 Gdf9条件性敲除小鼠的卵母细胞mRNA表达谱。