Degraded mangrove habitat site of Indian Sundarbans soil microbiome Metagenome. Degraded mangrove habitat site of Indian Sundarbans soil microbiome

This study was initiated to find out the absolute abundance of presence of different bacterial community in these degraded mangrove habitat sites soil of Indian Sundarbans. It was done through isolation of soil DNA from the respective sites and amplicon database generation through16S (V3-V4) rRNA Sequencing using Illumina MiSeq.Comparison of these sites information with that of pristine mangrove habitat site soil and restored or semi-restored mangrove habitat site soil of Indian Sundarbans might result into identification of some particular bacterial community abundances which could be used as metrics of evaluation restoration of mangrove site soil in future studies.

本研究旨在探究印度孙德尔本斯（Indian Sundarbans）退化红树林生境土壤中不同细菌群落的绝对丰度。实验通过从各对应采样位点分离土壤总DNA，采用Illumina MiSeq测序平台完成16S（V3-V4）核糖体RNA（rRNA）测序，进而构建扩增子数据库。将该退化生境位点的土壤数据与印度孙德尔本斯原生红树林生境土壤以及恢复或半恢复红树林生境土壤的相关信息进行对比分析，有望鉴定出具备指示价值的特定细菌群落丰度特征，该特征可在未来研究中作为红树林生境土壤修复效果的评估指标。