The expression profile in EA.hy926 cells after exposure to hypoxic conditions was evaluated by RNA-sequencing.

Hypoxia is an imbalance between oxygen supply and demand that occurs in embryonic development, as well as in pathological situations such as cancer, ischemic heart disease, and wound healing. To cope with decreased oxygen concentrations, a series of biological processes that endothelial cells participate in such as angiogenesis, are transcriptionally activated. In this work, we performed RNA-sequencing to profile activated genes in EA.hy926 cells after 8 hours of hypoxia, to reveal the changes of gene expression profile in endothelial cells under hypoxia. RNA-sequencing analysis was performed to identify genes whose expression in EA.hy926 cells changed significantly after 8 h of hypoxia.

缺氧（Hypoxia）是指在胚胎发育过程中，以及癌症、缺血性心脏病、伤口愈合等病理状态下，出现的氧供给与需求失衡现象。为应对氧浓度降低，内皮细胞（endothelial cells）参与的一系列生物学过程（如血管生成（angiogenesis））会被转录激活。本研究对缺氧处理8小时后的EA.hy926细胞进行RNA测序（RNA-sequencing），旨在解析缺氧条件下内皮细胞的基因表达谱变化，并筛选出该细胞系中缺氧处理8小时后表达发生显著改变的基因。