Gene expression pattern of synovial cells from inflammatory and normal areas of osteoarthritis synovial membrane

The aim of this study was to compare the gene expression pattern of synovial cells from inflammatory (I) or normal/reactive (N/R) areas of a synovial membrane harvested from the same osteoarthritis (OA) patient. This study is the first to identify different expression pattern between two areas of the synovial membrane in the same patient. These differences concern several key pathways involved in OA pathogenesis (inflammation, cartilage metabolism, Wnt signaling and angiogenesis). This analysis also provides interesting information regarding new potent intermediates as potentiel targets for the future therapeutic. Synovial tissues were obtained from 12 knee OA patients at the time of total knee replacement. The inflammatory status of the synovial membrane was characterized according to macroscopic criteria and sorted as N/R and I. Biopsies were cultured separately for 7 days. Microarray gene expression profiling between N/R and I areas was performed.

本研究旨在对比取自同一骨关节炎（osteoarthritis, OA）患者的滑膜组织中，炎症区域（I）与正常/反应性区域（N/R）内滑膜细胞的基因表达模式。本研究首次在同一患者的滑膜组织两个区域间鉴定出差异表达的基因谱。这些差异涉及骨关节炎发病机制相关的多条核心通路，包括炎症反应、软骨代谢、Wnt信号通路与血管生成。本分析还可为未来治疗的潜在靶点提供有关新型强效中间产物的有价值参考信息。研究从12名接受全膝关节置换术的膝骨关节炎患者术中获取滑膜组织，依据宏观标准对滑膜组织的炎症状态进行评估并分型为N/R与I区域。将两组活检标本分别体外培养7天，随后开展N/R与I区域间的微阵列基因表达谱分析。