Development of a qPCR assay and tremabiome deep amplicon sequencing for fluke species differentiation in the UK livestock

This dataset supports the development of a q deep amplicon sequencing approach tremabiome for the identification and differentiation of fluke species infecting livestock in the United Kingdom. It includes Illumina MiSeq paired-end sequencing data generated from genomic DNA extracted from Fasciola hepatica adult worms, faecal fluke eggs, and mock mixtures of eggs representing known species of different fluke compositions. Samples were collected from naturally infected cattle and sheep, and the targeted amplification of ITS2 region enables accurate species-level diagnosis. The dataset contributes to improving molecular diagnostics, understanding species co-infections, and informing parasite control strategies in UK livestock.

本数据集支持开发用于鉴定和区分英国感染家畜体内吸虫物种的定量深度扩增子测序吸虫组（tremabiome）研究方法。数据集包含来自以下样本的Illumina MiSeq成对末端测序数据：提取自肝片吸虫（Fasciola hepatica）成虫、粪便吸虫卵，以及代表不同吸虫物种组成的已知物种卵模拟混合物的基因组DNA。样本采集自自然感染的牛和绵羊，通过对内部转录间隔区2（ITS2）的靶向扩增可实现精准的物种水平诊断。本数据集有助于改进分子诊断技术、解析物种共感染情况，并为英国家畜的寄生虫防控策略制定提供科学支撑。