Influence of the ecto-nucleotidase inhibitor APCP and ADA on AMP-mediated effects.

Cells were pre-incubated for 20 min with the ecto-nucleotidase inhibitor APCP (5′-(alpha,beta-methylene) diphosphate) at a concentration of 10−6 M. Ca2+ transients were analyzed, and the ratio after stimulation with AMP at a concentration of 10−4 M for 10 s is given. Data are means ± SEM (n = 3). Actin polymerization after stimulation with AMP for 25 s was measured and calculated as the ratio between the medium control and stimulated cells. Data are means ± SEM (n = 3). The chemotactic index after stimulation with AMP at 10−5 M was calculated as the ratio between stimulated cells and cells incubated with medium. Data are means ± SE (n = 3).#p*p细胞经浓度为10^-6 M的胞外核苷酸酶抑制剂APCP（5′-(α,β-亚甲基)二磷酸）预孵育20分钟。对钙瞬变进行分析，本数据集给出了经浓度为10^-4 M的腺苷一磷酸（AMP）刺激10秒后的比值。数据以平均值±标准误（Standard Error of the Mean, SEM）表示（n=3）。经AMP刺激25秒后的肌动蛋白聚合水平经检测，并以培养基对照组与刺激组细胞的比值进行计算。数据以平均值±标准误（SEM）表示（n=3）。经10^-5 M的AMP刺激后的趋化指数以刺激组细胞与培养基孵育组细胞的比值计算得到。数据以平均值±标准误（Standard Error, SE）表示（n=3）。#p*p