RNA-seq of Arabidopsis thaliana seedlings exposed to different communities of commensal bacteria [SSC]. RNA-seq of Arabidopsis thaliana seedlings exposed to different communities of commensal bacteria [SSC]

This study evaluates the transcriptome of Arabidopsis thaliana seedlings exposed to communities of suppressors (SynCom5-S1) and non-suppressors (SynCom5-NS1) of the plant immune system Overall design: Arabidopsis seeds (wild-type Col-0) were germinated on Johnson medium and grown for 7 days in the vertical position. Plants were then transferred to media containing SynCom5-S1, SynCom5-NS1 (10E5 c.f.u / mL per strain), or no bacteria. Plants were grown in a growth chamber in a 15-h dark/9-h light regime (21°C day /18°C night). Seedlings were harvested twelve days after treatment. The experiment includes six biological replicates from two independent experiments.

本研究对暴露于植物免疫系统抑制菌群（SynCom5-S1）与非抑制菌群（SynCom5-NS1）的拟南芥（Arabidopsis thaliana）幼苗的转录组（transcriptome）进行评估。实验设计概述：将野生型Col-0拟南芥种子播种于约翰逊培养基（Johnson medium）上，于竖直培养条件下萌发并培养7天；随后将植株转接至含有SynCom5-S1、SynCom5-NS1（每菌株10^5菌落形成单位（colony-forming unit, CFU）/毫升）或无菌的培养基中。植株置于光照培养箱（growth chamber）内，采用15小时黑暗/9小时光照的光周期培养（昼温21℃、夜温18℃）。于处理后12天收获幼苗。本实验包含2次独立实验得到的6次生物学重复。