Cost Estimates for Pooled Sequencing Projects.

Cost estimates based on a 100 bp HiSeq paired-end run with Illumina's published reagent costs ($11,150 per flowcell) and an average throughput of 200 Gb per run. Sample preparation includes $75 per samples for library prep and $200 per sample for solution based capture. All calculations for pooled sequencing assume 20 individuals per pool.

本成本估算基于100碱基对（base pair，缩写bp）的HiSeq双端测序运行，所用参数为Illumina官方公布的试剂成本（每流动槽（flowcell）11150美元）与单次运行平均200 Gb的测序通量。样本制备成本包含每样本75美元的文库制备费用，以及每样本200美元的液相捕获（solution-based capture）费用。所有混合测序的成本核算均假设每个混合池包含20个个体。