Trancriptome analysis of honey bees (Apis mellifera) infected with Nosema ceranae. Apis mellifera

The microsporidia Nosema ceranae are intracellular parasites that proliferate in the midgut epithelial cells of honey bees (Apis mellifera). To analyze the pathological effects of those microsporidia, we orally infected honey bee workers 7 days after their emergence. Bees were flash frozen 15 days after the infection. Then, the effects on the gut ventriculi were analyzed and compared to non-infected (control) bees. Overall design: Comparisons of control vs Nosema ceranae bees

蜜蜂微孢子虫（Nosema ceranae）是一类细胞内寄生虫，可在西方蜜蜂（Apis mellifera）的中肠上皮细胞内增殖。为分析该类微孢子虫的致病效应，本研究于工蜂羽化7天后对其进行经口感染，并于感染后15天将受试蜜蜂快速冷冻。随后对受试蜜蜂的中肠进行相关效应分析，并与未感染（对照）组蜜蜂进行对比。实验整体设计：未感染对照组与感染Nosema ceranae实验组的比较