CD34+ and Treatment with UTP. Homo sapiens

Hematopoietic stem cell (HSC) untreated and treated with UTP 10uM at 1, 6 and 24 h Keywords: Time couse Overall design: Highly purified CD34+ cells from 6 healthy donors were seeded at 1000000 cells/ml in serum free medium (EX vivo 15) w/o cytokines and treated with 10 uM UTP for 1, 6 and 24 hours. As a control, CD34+ untreated cells were maintened in the same culture conditions for the same times. RNAs originating from the 6 donors were pooled in order to obtain at least 2 mg per sample (untreated and UTP-treated respectively).

造血干细胞（Hematopoietic stem cell，HSC）未经处理及以10μM UTP处理1、6和24小时的样本。关键词：时间进程（原文为Time couse，系time course笔误）。整体实验设计：从6名健康供体中获取的高纯度CD34+细胞以1×10^6 cells/mL的密度接种于不含细胞因子的无血清培养基（EX vivo 15）中，分别以10μM UTP处理1、6及24小时；对照组为在相同培养条件下培养对应时长的未处理CD34+细胞。将6名供体来源的RNA混合，以确保未处理组与UTP处理组的每个样本至少获得2mg RNA。