Data_Sheet_1_Silicone tags as an effective method of monitoring environmental contaminant exposures in a geographically diverse sample of dogs from the Dog Aging Project.pdf

IntroductionCompanion animals offer a unique opportunity to investigate risk factors and exposures in our shared environment. Passive sampling techniques have proven effective in capturing environmental exposures in dogs and humans. MethodsIn a pilot study, we deployed silicone monitoring devices (tags) on the collars of a sample of 15 dogs from the Dog Aging Project Pack cohort for a period of 120 h (5 days). We extracted and analyzed the tags via gas chromatography–mass spectrometry for 119 chemical compounds in and around participants’ homes. ResultsAnalytes belonging to the following chemical classes were detected: brominated flame retardants (BFRs), organophosphate esters (OPEs), polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), pesticides, phthalates, and personal care products. The types and amounts of analytes detected varied substantially among participants. DiscussionData from this pilot study indicate that silicone dog tags are an effective means to detect and measure chemical exposure in and around pet dogs’ households. Having created a sound methodological infrastructure, we will deploy tags to a geographically diverse and larger sample size of Dog Aging Project participants with a goal of further assessing geographic variation in exposures.引言 伴侣动物为探究人类共享环境中的风险因素与暴露情形提供了独特契机。被动采样技术已被证实可有效捕获犬类与人类的环境暴露物。 方法 本预实验中，我们从犬衰老项目（Dog Aging Project）Pack队列中选取15只犬作为样本，将硅胶监测装置（silicone monitoring devices，下称标签）佩戴于其项圈，为期120小时（5天）。随后通过气相色谱-质谱联用法对标签进行萃取与分析，以检测研究参与者住宅内外的119种化学物质。 结果 本次检测到了以下几类化学物质的分析物：溴化阻燃剂（brominated flame retardants, BFRs）、有机磷酸酯（organophosphate esters, OPEs）、多环芳烃（polycyclic aromatic hydrocarbons, PAHs）、多氯联苯（polychlorinated biphenyls, PCBs）、农药、邻苯二甲酸盐以及个人护理用品。不同研究参与者所检测到的分析物种类与含量差异显著。 讨论 本预实验的数据表明，硅胶犬用标签是检测并量化伴侣犬住宅内外化学暴露情况的有效手段。在搭建起完善的方法学基础后，我们将把标签部署至犬衰老项目中地域分布更广、样本量更大的参与者群体，以期进一步评估暴露情况的地域差异。